SOX2 ELISA Kit for Chicken(Gallus gallus) | EIAab.comSOX2 ELISA Kit for Chicken(Gallus gallus) | EIAab.com

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SOX2 (Gene name),

Transcription factor SOX-2 (Protein name ), SOX2_CHICK from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Related Databases

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Gene name:

SOX2;

Protein name:

Transcription factor SOX-2(cSox2);

Alternative:

delta EF2a;

Organism:

Chicken (Gallus gallus).

General Annotation

Sub Unit:

N/A

Function:

Transcriptional activator. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. In cooperation with other transcription factors, binds to the promoter sequence of the crystallin gene to activate transcription in the lens. Binds to the consensus DNA sequence 5'-TCATTGTTGTTG-3'.

Subcellular Location:

Nucleus

Protein Attributes:

Sequence length:

315

Sequence:

50:

MYNMMETELK | PPAPQQTSGG | GTGNSNSAAN | NQKNSPDRVK | RPMNAFMVWS |

100:

RGQRRKMAQE | NPKMHNSEIS | KRLGAEWKLL | SEAEKRPFID | EAKRLRALHM |

150:

KEHPDYKYRP | RRKTKTLMKK | DKYTLPGGLL | APGTNTMTTG | VGVGATLGAG |

200:

VNQRMDSYAH | MNGWTNGGYG | MMQEQLGYPQ | HPGLNAHNAA | QMQPMHRYDV |

250:

SALQYNSMTS | SQTYMNGSPT | YSMSYSQQGT | PGMALGSMGS | VVKTESSSSP |

300:

PVVTSSSHSR | APCQAGDLRD | MISMYLPGAE | VPEPAAPSRL | HMSQHYQSAP |

315:

VPGTAINGTL | PLSHM

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

Pfam:

String:

UniGene:

KEGG:

SMR:

Uniprot:

FOR

ELISA Kit for Chicken cSox2

Cat.:

E0423h

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Packing:

96T

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ELISA Kit for Chicken cSox2

Cat.:

E0423m

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96T

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ELISA Kit for Chicken cSox2

Cat.:

E0423c

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MSDS:

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Packing:

96T

CLIA Kit for Chicken cSox2

Cat.:

U0423c

Price:

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MSDS:

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Packing:

96T

CLIA Kit for Chicken cSox2

Cat.:

U0423m

Price:

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MSDS:

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Packing:

96T

CLIA Kit for Chicken cSox2

Cat.:

U0423h

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96T

Range:

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Polyclonal Antibody for Chicken cSox2

Cat.:

P0423Rb-h

Price:

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Packing:

40ug/0.2ml

Polyclonal Antibody for Chicken cSox2

Polyclonal Antibody for Chicken cSox2

Cat.:

P0423Rb-m

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Packing:

40ug/0.2ml

Monoclonal Antibody for Chicken cSox2

Cat.:

M0423h

Price:

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Packing:

40ug

Monoclonal Antibody for Chicken cSox2

Cat.:

M0423c

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Packing:

40ug

Monoclonal Antibody for Chicken cSox2

Cat.:

M0423m

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Packing:

40ug

Protein for Chicken cSox2

Cat.:

R0423h

Price:

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Packing:

1mg

Purity:

>90%

Protein for Chicken cSox2

Protein for Chicken cSox2

R&D Technical Data

s

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

s

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Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.9%

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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Recovery

Recovery was determined by spiking various levels of SOX2 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	91-104
Plasma	98	94-107

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Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SOX2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	90-103%	92-102%	89-106%	93-100%
EDTA plasma(n=5)	92-104%	90-107%	95-101%	94-104%
heparin plasma(n=5)	88-107%	94-103%	93-102%	91-106%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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References

1.

"Embryonic expression of the chicken Sox2, Sox3 and Sox11 genes suggests an interactive role in neuronal development."
Uwanogho D. , Rex M. , Cartwright E.J. , Pearl G. , Healy C. , Scotting P.J. , Sharpe P.T.
Mech. Dev.49:23-36(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PUTATIVE FUNCTION;TISSUE SPECIFICITY;DEVELOPMENTAL STAGE
tissue: Embryo.
2.

"Involvement of SOX proteins in lens-specific activation of crystallin genes."
Kamachi Y. , Sockanathan S. , Liu Q. , Breitman M. , Lovell-Badge R. , Kondoh H.
EMBO J.14:3510-3519(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];FUNCTION;DNA-BINDING;SUBCELLULAR LOCATION;TISSUE SPECIFICITY
strain: White leghorn.
tissue: Brain.
3.

"Functional analysis of chicken Sox2 enhancers highlights an array of diverse regulatory elements that are conserved in mammals."
Uchikawa M. , Ishida Y. , Takemoto T. , Kamachi Y. , Kondoh H.
Dev. Cell4:509-519(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
tissue: Liver.
4.

"Vertebrate neurogenesis is counteracted by Sox1-3 activity."
Bylund M. , Andersson E. , Novitch B.G. , Muhr J.
Nat. Neurosci.6:1162-1168(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION

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